Anti-Aconitase 2 antibody (ab129957)
Key features and details
- Rabbit polyclonal to Aconitase 2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Aconitase 2 antibody
See all Aconitase 2 primary antibodies -
Description
Rabbit polyclonal to Aconitase 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
corresponding to Aconitase 2.
(Peptide available asab169564) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Brain; Human Liver; Human Kidney; Human Heart; Human Heart Mitochondrial; Mouse Brain; Rat Brain.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatHumanAll applications RabbitHorseChickenCowDogPigChimpanzeeZebrafishGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).Target
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Function
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. -
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2. -
Sequence similarities
Belongs to the aconitase/IPM isomerase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 374009 Chicken
- Entrez Gene: 280976 Cow
- Entrez Gene: 474487 Dog
- Entrez Gene: 101126782 Gorilla
- Entrez Gene: 100055803 Horse
- Entrez Gene: 50 Human
- Entrez Gene: 11429 Mouse
- Entrez Gene: 396999 Pig
see all -
Alternative names
- ACO 2 antibody
- ACO2 antibody
- ACON_HUMAN antibody
see all
Images
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Western blot - Anti-Aconitase 2 antibody (ab129957)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Aconitase 2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab129957 observed at 85 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab129957 was shown to recognize Aconitase 2 in wild-type HAP1 cells as signal was lost at the expected MW in Aconitase 2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Aconitase 2 knockout samples were subjected to SDS-PAGE. ab129957 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Aconitase 2 antibody (ab129957)All lanes : Anti-Aconitase 2 antibody (ab129957) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lane 5 : Human Heart Mitochondrial Lysate
Lane 6 : Brain (Mouse) Tissue Lysate
Lane 7 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 85 kDa
Additional bands at: 35 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129957 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (1)
ab129957 has been referenced in 1 publication.
- Metodiev MD et al. Mutations in the tricarboxylic acid cycle enzyme, aconitase 2, cause either isolated or syndromic optic neuropathy with encephalopathy and cerebellar atrophy. J Med Genet 51:834-8 (2014). PubMed: 25351951
Images
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Western blot - Anti-Aconitase 2 antibody (ab129957)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Aconitase 2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab129957 observed at 85 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab129957 was shown to recognize Aconitase 2 in wild-type HAP1 cells as signal was lost at the expected MW in Aconitase 2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Aconitase 2 knockout samples were subjected to SDS-PAGE. ab129957 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Aconitase 2 antibody (ab129957)All lanes : Anti-Aconitase 2 antibody (ab129957) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lane 5 : Human Heart Mitochondrial Lysate
Lane 6 : Brain (Mouse) Tissue Lysate
Lane 7 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 85 kDa
Additional bands at: 35 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129957 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
