Anti-TNF alpha antibody [NF-7] (ab9809)
![Anti-TNF alpha antibody [NF-7] (ab9809)](https://www.abcam.com/ps/products/9/ab9809/Images/ab9809-17472-anti-tnf-alpha-antibody-nf-7-flow-cytometry.jpg "Anti-TNF alpha antibody [NF-7] (ab9809)")
Key features and details
- Mouse monoclonal [NF-7] to TNF alpha
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TNF alpha antibody [NF-7]
See all TNF alpha primary antibodies -
Description
Mouse monoclonal [NF-7] to TNF alpha -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein (Human).
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Positive control
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General notes
This antibody can be used in two-site immunoassay for the determination of human TNF alpha. It recognizes a different epitope than the monoclonal antibody ab9813. The two-site immunoassay can be used to determine TNF alpha concentration in the cultures of human peripheral blood mononuclear cells. Can also be used to neutralize TNF alpha cytotoxic activity in vitro. At a concentration of 2.5 µg/ml, antibody ab9809 causes 50% inhibition of TNF alpha mediated cytotoxicity on L929 cells.
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR151594-8 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR151594-8 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
This antibody can be used in two-site immunoassay for the determination of human TNF alpha. It recognizes a different epitope than the monoclonal antibody ab9813. The two-site immunoassay can be used to determine TNF alpha concentration in the cultures of human peripheral blood mononuclear cells. Can also be used to neutralize TNF alpha cytotoxic activity in vitro. At a concentration of 2.5 µg/ml, antibody ab9809 causes 50% inhibition of TNF alpha mediated cytotoxicity on L929 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
This antibody can be used in two-site immunoassay for the determination of human TNF alpha. It recognizes a different epitope than the monoclonal antibody ab9813. The two-site immunoassay can be used to determine TNF alpha concentration in the cultures of human peripheral blood mononuclear cells. Can also be used to neutralize TNF alpha cytotoxic activity in vitro. At a concentration of 2.5 µg/ml, antibody ab9809 causes 50% inhibition of TNF alpha mediated cytotoxicity on L929 cells. -
Clonality
Monoclonal -
Clone number
NF-7 -
Myeloma
NS0/1 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
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Flow Cytometry - Anti-TNF alpha antibody [NF-7] (ab9809)Overlay histogram showing THP1 cells stained with ab9809 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9809, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
