Anti-Aconitase 2 antibody (ab228923)
Key features and details
- Rabbit polyclonal to Aconitase 2
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Aconitase 2 antibody
See all Aconitase 2 primary antibodies -
Description
Rabbit polyclonal to Aconitase 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseIP HumanWB MouseRatHuman
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Immunogen
Recombinant fragment within Human Aconitase 2 (internal sequence). The exact sequence is proprietary.
Database link: Q99798 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts; Rat brain extract; Mouse brain lysate; Jurkat whole cell lysate. IP: HeLa whole cell extract. IHC-P: Mouse liver, kidney, urinary bladder and intestine tissues. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Aconitase 2 antibody (ab228923)All lanes : Anti-Aconitase 2 antibody (ab228923) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-Aconitase 2 antibody (ab228923)Anti-Aconitase 2 antibody (ab228923) at 1/10000 dilution + Rat brain extract at 50 µg
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-Aconitase 2 antibody (ab228923)Anti-Aconitase 2 antibody (ab228923) at 1/10000 dilution + Mouse brain lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-Aconitase 2 antibody (ab228923)Anti-Aconitase 2 antibody (ab228923) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
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Immunoprecipitation - Anti-Aconitase 2 antibody (ab228923)Aconitase 2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg ab228923. Western blot was performed from the immunoprecipitate using ab228923. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in HeLa whole cell extract.
Lane 2: ab228923 IP in HeLa whole cell extract.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody (ab228923)Paraffin-embedded mouse liver tissue stained for Aconitase 2 using ab228923 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody (ab228923)2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Aconitase 2 (green) using ab228923 at 1/1000 dilution in ICC/IF.
Red: A mitochondria tracker.
Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody (ab228923)Paraffin-embedded mouse kidney tissue stained for Aconitase 2 using ab228923 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody (ab228923)Paraffin-embedded mouse urinary bladder tissue stained for Aconitase 2 using ab228923 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody (ab228923)Paraffin-embedded mouse intestine tissue stained for Aconitase 2 using ab228923 at 1/500 dilution in immunohistochemical analysis.
