Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] - BSA and Azide free (ab249743)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7519(2)] to DNA Polymerase lambda/Polk - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] - BSA and Azide free
    See all DNA Polymerase lambda/Polk primary antibodies

  • Description

    Rabbit monoclonal [EPR7519(2)] to DNA Polymerase lambda/Polk - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and A549 cell lysates.

  • General notes

    ab249743 is the carrier-free version of ab172974 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249743 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as DNA Polymerase lambda

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] - BSA and Azide free (ab249743)
    Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] - BSA and Azide free (ab249743)
    All lanes : Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] (ab172974) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : POLL knockout HeLa cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab172974).

    Lanes 1-3: Merged signal (red and green). Green - ab172974 observed at 75 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab172974 Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] was shown to specifically react with DNA Polymerase lambda/Polk in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264754 (knockout cell lysate ab258595) was used. Wild-type and DNA Polymerase lambda/Polk knockout samples were subjected to SDS-PAGE. ab172974 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

     

  • Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] - BSA and Azide free (ab249743)
    Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] - BSA and Azide free (ab249743)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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