Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] - BSA and Azide free (ab249045)
![Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] - BSA and Azide free (ab249045)](https://www.abcam.com/ps/products/249/ab249045/Images/ab249045-2-anti-cytochrome-p450-1a2-antibody-epr6138-2-flowcytometry-mcf7-human.jpg "Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] - BSA and Azide free (ab249045)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6138(2)] to Cytochrome P450 1A2 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Human
Overview
-
Product name
Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] - BSA and Azide free
See all Cytochrome P450 1A2 primary antibodies -
Description
Rabbit monoclonal [EPR6138(2)] to Cytochrome P450 1A2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Caco2, HepG2, HeLa and A549 cell lysates. ICC/IF: HeLa cells. Flow Cyt: MCF7 cells.
-
General notes
ab249045 is the carrier-free version of ab151728 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249045 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6138(2) -
Isotype
IgG -
Research areas
Images
-
Flow Cytometry - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] - BSA and Azide free (ab249045)Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Cytochrome P450 1A2 with purified ab151728 at 1/200 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151728) -
![Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] - BSA and Azide free (ab249045)](https://www.abcam.com/ps/products/249/ab249045/Images/ab249045-1-anti-cytochrome-p450-1a2-antibody-epr6138-2-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] - BSA and Azide free (ab249045)Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytochrome P450 1A2 with purified ab151728 at 1/200 dilution (9.4 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151728) -
![Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] - BSA and Azide free (ab249045)](https://www.abcam.com/ps/products/249/ab249045/Images/ab249045-3-benefits-of-recombinant-antibodies.png)
Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] - BSA and Azide free (ab249045)
