Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24135-98] to Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 - BSA and Azide free
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free
    See all Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 primary antibodies

  • Description

    Rabbit monoclonal [EPR24135-98] to Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-Fr,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human Hippocampus, brain, testis, kidney tissue lysates, A549, MCF7, Neuro-2a, Mouse testis, Rat hippocampus, brain, testis and kidney lysates. ICC/IF: A549 and mouse primary neuron, rat primary neuron cells

  • General notes

    ab280367 is the carrier-free version of ab259859.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)
    Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)
    All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] (ab259859) at 1/1000 dilution

    Lane 1 : Human Hippocampus tissue lysate
    Lane 2 : Human brain tissue lysate
    Lane 3 : Human testis tissue lysate
    Lane 4 : Human kidney tissue lysate
    Lane 5 : Human liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    This data was developed using ab259859, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The observed MW is consistent with what has been described in the literature (PMID:30543688).

    Low expression: kidney, liver (PMID:20920278).

    Exposure time: Lane 1: 10 seconds

    Lane 2-5: 26 seconds

  • Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)
    Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)
    All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] (ab259859) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    This data was developed using ab259859, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The observed MW and expression pattern is consistent with what has been described in the literature (PMID:30543688, PMID:28878681).

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: Lane 1: 3 minutes

    Lane 2: 26 seconds

     

  • Immunocytochemistry - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)
    Immunocytochemistry - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)

    This data was developed using ab259859, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 cells labelling Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 with ab259859 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A549 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)
    Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)
    All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] (ab259859) at 1/1000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
    Lane 2 : Mouse testis tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    This data was developed using ab259859, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The observed MW is consistent with what has been described in the literature (PMID:30543688).

    Low expression: kidney, liver (PMID:20920278).

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: 3 minutes

     

  • Immunocytochemistry - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)
    Immunocytochemistry - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)

    This data was developed using ab259859, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 with ab259859 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)
    Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)
    All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] (ab259859) at 1/1000 dilution

    Lane 1 : Rat hippocampus tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat testis tissue lysate
    Lane 4 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    This data was developed using ab259859, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The observed MW is consistent with what has been described in the literature (PMID:30543688).

    Low expression: kidney (PMID:20920278).

    Samples (except lane1) are non-boiled as boiling may cause protein aggregates.

    Exposure time: 26 seconds

  • Immunocytochemistry - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)
    Immunocytochemistry - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)

    This data was developed using ab259859, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 with ab259859 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in rat primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)
    Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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