Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free
See all Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 primary antibodies
Description

Rabbit monoclonal [EPR24135-98] to Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-Fr,IHC-P or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human Hippocampus, brain, testis, kidney tissue lysates, A549, MCF7, Neuro-2a, Mouse testis, Rat hippocampus, brain, testis and kidney lysates. ICC/IF: A549 and mouse primary neuron, rat primary neuron cells
General notes
ab280367 is the carrier-free version of ab259859.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C.
Storage buffer

Constituent: 100% PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24135-98
Isotype

IgG
Research areas
- Neuroscience
- Processes

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)

All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] (ab259859) at 1/1000 dilution

Lane 1 : Human Hippocampus tissue lysate
Lane 2 : Human brain tissue lysate
Lane 3 : Human testis tissue lysate
Lane 4 : Human kidney tissue lysate
Lane 5 : Human liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

Predicted band size: 53 kDa
Observed band size: 53 kDa

This data was developed using ab259859, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The observed MW is consistent with what has been described in the literature (PMID:30543688).

Low expression: kidney, liver (PMID:20920278).

Exposure time: Lane 1: 10 seconds

Lane 2-5: 26 seconds
Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)

All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] (ab259859) at 1/1000 dilution

Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 53 kDa
Observed band size: 53 kDa

This data was developed using ab259859, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The observed MW and expression pattern is consistent with what has been described in the literature (PMID:30543688, PMID:28878681).

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time: Lane 1: 3 minutes

Lane 2: 26 seconds
Immunocytochemistry - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)

This data was developed using ab259859, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 cells labelling Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 with ab259859 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A549 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)

All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] (ab259859) at 1/1000 dilution

Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
Lane 2 : Mouse testis tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 53 kDa
Observed band size: 53 kDa

This data was developed using ab259859, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The observed MW is consistent with what has been described in the literature (PMID:30543688).

Low expression: kidney, liver (PMID:20920278).

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time: 3 minutes
Immunocytochemistry - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)

This data was developed using ab259859, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 with ab259859 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)

All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] (ab259859) at 1/1000 dilution

Lane 1 : Rat hippocampus tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat testis tissue lysate
Lane 4 : Rat kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 53 kDa
Observed band size: 53 kDa

This data was developed using ab259859, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The observed MW is consistent with what has been described in the literature (PMID:30543688).

Low expression: kidney (PMID:20920278).

Samples (except lane1) are non-boiled as boiling may cause protein aggregates.

Exposure time: 26 seconds
Immunocytochemistry - Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)

This data was developed using ab259859, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 with ab259859 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in rat primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody [EPR24135-98] - BSA and Azide free (ab280367)