Anti-VEGF Receptor 3 antibody [EPR22293-14] - BSA and Azide free (ab243565)
![Anti-VEGF Receptor 3 antibody [EPR22293-14] - BSA and Azide free (ab243565)](https://www.abcam.com/ps/products/243/ab243565/Images/ab243565-333738-ab243565.jpg "Anti-VEGF Receptor 3 antibody [EPR22293-14] - BSA and Azide free (ab243565)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22293-14] to VEGF Receptor 3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-VEGF Receptor 3 antibody [EPR22293-14] - BSA and Azide free
See all VEGF Receptor 3 primary antibodies -
Description
Rabbit monoclonal [EPR22293-14] to VEGF Receptor 3 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEL whole cell lysate.
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General notes
Ab243565 is the carrier-free version of ab243232. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab243565 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22293-14 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-VEGF Receptor 3 antibody [EPR22293-14] - BSA and Azide free (ab243565)All lanes : Anti-VEGF Receptor 3 antibody [EPR22293-14] (ab243232) at 1/1000 dilution
Lane 1 : HEL (human erythroleukemia erythroblast cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 146 kDa
Observed band size: 125,175,80 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a higher sensitivity ECL substrate.
The molecular weight observed is consistent with the literatures (PMID: 18538738, PMID: 7692369, PMID: 14648657).
Negative control: MCF7 (PMID: 18538738).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243232).
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![Anti-VEGF Receptor 3 antibody [EPR22293-14] - BSA and Azide free (ab243565)](https://www.abcam.com/ps/products/243/ab243565/Images/ab243565-2-benefits-of-recombinant-antibodies.png)
Anti-VEGF Receptor 3 antibody [EPR22293-14] - BSA and Azide free (ab243565)
