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Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)

Overview

  • Product name

    Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICCmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Ion Exchange Chromatography

  • Clonality

    Monoclonal

  • Clone number

    R313-MouseIgG2c

  • Isotype

    IgG2c

Images

  • Immunocytochemistry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)
    Immunocytochemistry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Confocal image showing no staining in MOLT-4 cells.

    Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

  • Flow Cytometry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)
    Flow Cytometry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labelling Mouse IgG2c with ab280756 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

     

  • Immunocytochemistry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)
    Immunocytochemistry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Confocal image showing no staining in NIH/3T3 cells.

    Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

  • Flow Cytometry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)
    Flow Cytometry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast, Left) / PC-12 (Rat adrenal gland pheochromocytoma, Right) cells labelling Mouse IgG2c with ab280756 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)
    Immunocytochemistry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NPC-12 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Confocal image showing no staining in PC-12 cells.

    Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

  • Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)
    Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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