Antibodies, Proteins, Kits and Reagents for Life Science

Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)

Product name

Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control
Tested applications

Suitable for: Flow Cyt (Intra), ICCmore details
Unsuitable for: IHC-P
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

The details of the immunogen for this antibody are not available.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Ion Exchange Chromatography
Clonality

Monoclonal
Clone number

R313-MouseIgG2c
Isotype

IgG2c

Immunocytochemistry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Confocal image showing no staining in MOLT-4 cells.

Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.

Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
Flow Cytometry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labelling Mouse IgG2c with ab280756 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti mouse IgG (Alexa Fluor^® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Confocal image showing no staining in NIH/3T3 cells.

Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.

Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
Flow Cytometry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast, Left) / PC-12 (Rat adrenal gland pheochromocytoma, Right) cells labelling Mouse IgG2c with ab280756 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor^® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NPC-12 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Confocal image showing no staining in PC-12 cells.

Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.

Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)

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