Biotin Anti-Listeria monocytogenes antibody (ab20766)
Key features and details
- Biotin Rabbit polyclonal to Listeria monocytogenes
- Suitable for: Flow Cyt, ELISA, IHC-Fr
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Listeria monocytogenes antibody
See all Listeria monocytogenes primary antibodies -
Description
Biotin Rabbit polyclonal to Listeria monocytogenes -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cyt, ELISA, IHC-Frmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Tissue, cells or virus corresponding to Listeria monocytogenes Listeria monocytogenes.
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General notes
IgG fraction covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Biotin Anti-Listeria monocytogenes antibody (ab20766) This image is a courtesy of Anonymous Abreview
ab20766 staining Listeria monocytogenes in mouse spleen tissue section from a mice infected with 1x10exp6 L. monocytogenes for 24h by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 4% paraformaldehyde for 2 hours at RT and then it was incubated ON in 30% Sucrose in PBS at 4°C, froze away in OCT/Tissuetek. The tissue samples were blocked with 1% 1:20 Cd16/32 at 200C for 30 minutes. The sample was incubated with primary antibody (1/250) in PBS at 200C for 1 hour. For detection Streptavidin-Cy3 was used at dilution at 1:500 in PBS for 30 min at 20°C.
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Flow Cytometry - Biotin Anti-Listeria monocytogenes antibody (ab20766) This image is courtesy of an anonymous AbreviewFlow cytometry analysis of Listeria monocytogenes in culture, staining Listeria monocytogenes with ab20766.
The sample was incubated with the primary antibody (1/300 in PBS) for 30 minutes at 4°C. A Streptavidin-Peridinin Chlorophyll-a Protein conjugated with PerCP was used for detection.
Gating Strategy: FSC/SSC
