Recombinant Staphylococcus alpha Hemolysin protein (Active) (ab233724)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Endotoxin level: = 5.165 Eu/mg
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE, ELISA
-
Product name
Recombinant Staphylococcus alpha Hemolysin protein (Active) -
Biological activity
ab233724 is active in functional lysis assays with rabbit red blood cells.
EC50 ~0.015 µg/ml.
-
Purity
> 80 % SDS-PAGE. -
Endotoxin level
= 5.165 Eu/mg -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Predicted molecular weight
33 kDa -
Additional sequence information
Recombinant protein TAG-free
-
Preparation and Storage
- Alpha hemolysin
- Alpha HL
- Alpha toxin
Images
-
Functional Studies - Recombinant Staphylococcus alpha Hemolysin protein (Active) (ab233724)
Lysis of rabbit red blood cells by S. aureus Hla.
Red blood cell lysis was determined by absorbance at OD 416 nm after 30 min incubation at 37°C with Hla.
EC50 values were found to be 0.0262 µg/mL for lot 1805010 (blue circles) and 0.0322 µg/mL for previous lot 1805007 (green circles).
-
SDS-PAGE - Recombinant Staphylococcus alpha Hemolysin protein (Active) (ab233724)Lane 1: 5 ug.
Lane 2: 1 ug.
-
Western blot - Recombinant Staphylococcus alpha Hemolysin protein (Active) (ab233724)All lanes : Mouse anti-Alpha Toxin (6C12) monoclonal antibody at 1 µg/ml
Lane 1 :Recombinant Staphylococcus alpha Hemolysin protein (Active) (ab233724) at 0.005 µg
Lane 2 :Recombinant Staphylococcus alpha Hemolysin protein (Active) (ab233724) at 0.01 µg
Lane 3 :Recombinant Staphylococcus alpha Hemolysin protein (Active) (ab233724) at 0.05 µg
Secondary
All lanes : Anti-mouse IgG-HRP conjugate