Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

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Anti-TIMP3 antibody [EPR23943-149] (ab276134)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23943-149] to TIMP3
Suitable for: Flow Cyt, ICC, WB
Reacts with: Human

Product name

Anti-TIMP3 antibody [EPR23943-149]
See all TIMP3 primary antibodies
Description

Rabbit monoclonal [EPR23943-149] to TIMP3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MDA-MB-231 whole cell lysate; Human lung and placenta tissue lysates; Recombinant Human TIMP-3 Protein (aa24-211). Flow cyt: MDA-MB-231 cells. ICC: MDA-MB-231 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23943-149
Isotype

IgG
Research areas

Immunocytochemistry - Anti-TIMP3 antibody [EPR23943-149] (ab276134)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 cells labelling TIMP3 with ab276134 at 1/50 (9.16 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in MDA-MB-231 cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Western blot - Anti-TIMP3 antibody [EPR23943-149] (ab276134)

All lanes : Anti-TIMP3 antibody [EPR23943-149] (ab276134) at 1/1000 dilution

Lane 1 : Human placenta tissue lysate
Lane 2 : Human liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

Predicted band size: 24 kDa
Observed band size: 20 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8174111, 30894640).

Low expression tissue: human liver (PMID:8174111).

Exposure time: 125 seconds.
Flow Cytometry - Anti-TIMP3 antibody [EPR23943-149] (ab276134)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) cells labelling TIMP3 with ab276134 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-TIMP3 antibody [EPR23943-149] (ab276134)

Anti-TIMP3 antibody [EPR23943-149] (ab276134) at 1/1000 dilution + Recombinant Human TIMP-3 Protein, 20ng

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 24 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.
Western blot - Anti-TIMP3 antibody [EPR23943-149] (ab276134)

Anti-TIMP3 antibody [EPR23943-149] (ab276134) at 1/1000 dilution + Human lung tissue lysate at 20 µg

Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

Predicted band size: 24 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8174111, 30894640).

Exposure time: 92 seconds.
Western blot - Anti-TIMP3 antibody [EPR23943-149] (ab276134)

Anti-TIMP3 antibody [EPR23943-149] (ab276134) at 1/1000 dilution + MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 24 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8174111, 30894640).

Exposure time: 3 minutes.
Anti-TIMP3 antibody [EPR23943-149] (ab276134)

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