Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIMP3 antibody [EPR23943-149] (ab276134)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23943-149] to TIMP3
  • Suitable for: Flow Cyt, ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TIMP3 antibody [EPR23943-149]
    See all TIMP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR23943-149] to TIMP3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MDA-MB-231 whole cell lysate; Human lung and placenta tissue lysates; Recombinant Human TIMP-3 Protein (aa24-211). Flow cyt: MDA-MB-231 cells. ICC: MDA-MB-231 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-TIMP3 antibody [EPR23943-149] (ab276134)
    Immunocytochemistry - Anti-TIMP3 antibody [EPR23943-149] (ab276134)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 cells labelling TIMP3 with ab276134 at 1/50 (9.16 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in MDA-MB-231 cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-TIMP3 antibody [EPR23943-149] (ab276134)
    Western blot - Anti-TIMP3 antibody [EPR23943-149] (ab276134)
    All lanes : Anti-TIMP3 antibody [EPR23943-149] (ab276134) at 1/1000 dilution

    Lane 1 : Human placenta tissue lysate
    Lane 2 : Human liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 24 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8174111, 30894640).

    Low expression tissue: human liver (PMID:8174111).

     Exposure time: 125 seconds.

     

  • Flow Cytometry - Anti-TIMP3 antibody [EPR23943-149] (ab276134)
    Flow Cytometry - Anti-TIMP3 antibody [EPR23943-149] (ab276134)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) cells labelling TIMP3 with ab276134 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-TIMP3 antibody [EPR23943-149] (ab276134)
    Western blot - Anti-TIMP3 antibody [EPR23943-149] (ab276134)
    Anti-TIMP3 antibody [EPR23943-149] (ab276134) at 1/1000 dilution + Recombinant Human TIMP-3 Protein, 20ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

    Exposure time: 1 second.

     

  • Western blot - Anti-TIMP3 antibody [EPR23943-149] (ab276134)
    Western blot - Anti-TIMP3 antibody [EPR23943-149] (ab276134)
    Anti-TIMP3 antibody [EPR23943-149] (ab276134) at 1/1000 dilution + Human lung tissue lysate at 20 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 24 kDa
    Observed band size: 20 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8174111, 30894640).

    Exposure time: 92 seconds.

     

  • Western blot - Anti-TIMP3 antibody [EPR23943-149] (ab276134)
    Western blot - Anti-TIMP3 antibody [EPR23943-149] (ab276134)
    Anti-TIMP3 antibody [EPR23943-149] (ab276134) at 1/1000 dilution + MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa
    Observed band size: 20 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8174111, 30894640).

    Exposure time: 3 minutes.

     

  • Anti-TIMP3 antibody [EPR23943-149] (ab276134)
    Anti-TIMP3 antibody [EPR23943-149] (ab276134)

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