Nck overexpression 293T lysate (whole cell) (ab94184)
Overview
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Product name
Nck overexpression 293T lysate (whole cell)
See all Nck lysates -
General notes
ab94184 is a 293T cell transfected lysate in which Human Nck has been transiently over-expressed using a pCMV-Nck plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Similarity: Contains 1 SH2 domain. Contains 3 SH3 domains. PTM: Phosphorylated on Ser and Tyr residues.
Images
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SDS-PAGE - Nck overexpression 293T lysate (whole cell) (ab94184)ab94184 at 15 μg/lane on an SDS-PAGE gel
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Western blot - Nck overexpression 293T lysate (whole cell) (ab94184)All lanes : Anti-Nck antibody (ab55499) at 1/500 dilution
Lane 1 :Nck overexpression 293T lysate (whole cell) (ab94184)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
