Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DGKA antibody [EPR23176-101] - BSA and Azide free (ab269459)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23176-101] to DGKA - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-DGKA antibody [EPR23176-101] - BSA and Azide free
    See all DGKA primary antibodies

  • Description

    Rabbit monoclonal [EPR23176-101] to DGKA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, HeLa and Jurkat whole cell lysates; Human tonsil lysate. IHC-P: Human tonsil and Human spleen tissues. IP: Jurkat whole cell lysate.

  • General notes

    ab269459 is the carrier-free version of ab243647. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKA antibody [EPR23176-101] - BSA and Azide free (ab269459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKA antibody [EPR23176-101] - BSA and Azide free (ab269459)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling DGKA with ab243647 at 1/4000 dilution (0.163 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on T cells of human tonsil. The section was incubated with ab243647 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243647).

  • Immunoprecipitation - Anti-DGKA antibody [EPR23176-101] - BSA and Azide free (ab269459)
    Immunoprecipitation - Anti-DGKA antibody [EPR23176-101] - BSA and Azide free (ab269459)

    DGKA was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate with ab243647 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243647 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 10ug

    Lane 2: ab243647 IP in Jurkat whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243647 in Jurkat whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 40 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243647).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKA antibody [EPR23176-101] - BSA and Azide free (ab269459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKA antibody [EPR23176-101] - BSA and Azide free (ab269459)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling DGKA with ab243647 at 1/4000 dilution (0.163 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spleen is observed. The section was incubated with ab243647 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243647).

  • Anti-DGKA antibody [EPR23176-101] - BSA and Azide free (ab269459)
    Anti-DGKA antibody [EPR23176-101] - BSA and Azide free (ab269459)

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