Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11266(B)] to AKAP associated Sperm Protein/ROPN1L
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)]
    See all AKAP associated Sperm Protein/ROPN1L primary antibodies

  • Description

    Rabbit monoclonal [EPR11266(B)] to AKAP associated Sperm Protein/ROPN1L

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    within Human AKAP associated Sperm Protein/ROPN1L aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96C74

  • Positive control

    • Human testis lysate, Human testis tissue

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as AKAP associated Sperm Protein

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

    Immunohistochemical analysis of paraffin embedded Human testis tissue labeling AKAP associated Sperm Protein/ROPN1L with ab173298 at 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

    ab173298 showing +ve staining in Human normal testis tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

    ab173298 showing -ve staining in Human breast carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

    ab173298 showing -ve staining in Human cervical carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

    ab173298 showing -ve staining in Human skeletal muscle tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

    ab173298 showing -ve staining in Human normal brain tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)
    Western blot - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)
    Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298) at 1/1000 dilution + Human testis lysate at 10 µg

    Predicted band size: 26 kDa

  • Immunoprecipitation - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)
    Immunoprecipitation - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

    Immunoprecipitation. ab173298 at 1/1000 staining AKAP associated Sperm Protein/ROPN1L immunoprecipitated from Human tetis lysate using ab173297 at 1/10.

    Lane 2: 1X PBS (negative control) .


  • Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)
    Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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