Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µl 271 382 ₸

Order now and get it on Tuesday March 02, 2021

Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11266(B)] to AKAP associated Sperm Protein/ROPN1L
Suitable for: IP, IHC-P, WB
Reacts with: Human

Product name

Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)]
See all AKAP associated Sperm Protein/ROPN1L primary antibodies
Description

Rabbit monoclonal [EPR11266(B)] to AKAP associated Sperm Protein/ROPN1L
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

within Human AKAP associated Sperm Protein/ROPN1L aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q96C74
Positive control
- Human testis lysate, Human testis tissue
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as AKAP associated Sperm Protein

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11266(B)
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

Immunohistochemical analysis of paraffin embedded Human testis tissue labeling AKAP associated Sperm Protein/ROPN1L with ab173298 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

ab173298 showing +ve staining in Human normal testis tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

ab173298 showing -ve staining in Human breast carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

ab173298 showing -ve staining in Human cervical carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

ab173298 showing -ve staining in Human skeletal muscle tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

ab173298 showing -ve staining in Human normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298) at 1/1000 dilution + Human testis lysate at 10 µg

Predicted band size: 26 kDa
Immunoprecipitation - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

Immunoprecipitation. ab173298 at 1/1000 staining AKAP associated Sperm Protein/ROPN1L immunoprecipitated from Human tetis lysate using ab173297 at 1/10.

Lane 2: 1X PBS (negative control) . 
Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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