HRP Anti-Aurora A antibody [35C1] (ab199220)
![HRP Anti-Aurora A antibody [35C1] (ab199220)](https://www.abcam.com/ps/products/199/ab199220/Images/ab199220-246564-anti-aurora-a-antibody-35c1-hrp-western-blot.jpg "HRP Anti-Aurora A antibody [35C1] (ab199220)")
Key features and details
- HRP Mouse monoclonal [35C1] to Aurora A
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-Aurora A antibody [35C1]
See all Aurora A primary antibodies -
Description
HRP Mouse monoclonal [35C1] to Aurora A -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant full length protein corresponding to Human Aurora A.
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Positive control
- WB: Jurkat whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
35C1 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2b -
Research areas
Images
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Western blot - HRP Anti-Aurora A antibody [35C1] (ab199220)HRP Anti-Aurora A antibody [35C1] (ab199220) at 1/5000 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with 199220 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
