Human Apolipoprotein CII ELISA Kit (ab168549)
Key features and details
- Sensitivity: 0.013 µg/ml
- Range: 0.125 µg/ml - 4 µg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Apolipoprotein CII ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 6% Inter-assay Sample n Mean SD CV% Overall 10.2% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
> 0.013 µg/ml -
Range
0.125 µg/ml - 4 µg/ml -
Recovery
96 %
-
Assay time
4h 00m -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Apolipoprotein CII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein CII concentrations in plasma, serum, and cell culture supernatants.
An Apolipoprotein CII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein CII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein CII captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 40x Biotinylated Human Apolipoprotein CII Antibody 1 x 150µl Apolipoprotein CII Microplate (12 x 8 well strips) 1 unit Apolipoprotein CII Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
-
Alternative names
- APC2
- Apo-CII
- APOC II
see all -
Database links
- Entrez Gene: 344 Human
- Omim: 608083 Human
- SwissProt: P02655 Human
Images
-
Typical Standard Curve
Representsative Standard Curve using ab168549