Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)
![Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)](https://www.abcam.com/ps/products/110/ab110266/Images/ab110266-196280-anti-cytochrome-c-oxidase-subunit-vib-antibody-3f9d3d11af6-immunofluorescence.jpg "Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)")
Key features and details
- Mouse monoclonal [3F9D3D11AF6] to COX6B1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Overview
-
Product name
Anti-COX6B1 antibody [3F9D3D11AF6]
See all COX6B1 primary antibodies -
Description
Mouse monoclonal [3F9D3D11AF6] to COX6B1 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
-
Positive control
- Human, cow, rat and mouse heart. IF/ICC: HeLa cell line.
-
General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
This product was previously labelled as Cytochrome C Oxidase subunit VIb
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
Near homogeneity as judged by SDS-PAGE. ab110266 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
3F9D3D11AF6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)
ICC/IF image of ab110266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
![Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)](https://www.abcam.com/ps/products/110/ab110266/Images/ab110266-123458-anti-cytochrome-c-oxidase-subunit-vib-antibody-3f9d3d11af6-western-blot.jpg)
Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)All lanes : Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266) at 1 µg/ml
Lane 1 : Isolated mitochondria from Human Heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine Heart at 4 µg
Lane 3 : Isolated mitochondria from Rat Heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse Heart at 10 µg
Predicted band size: 10 kDa
