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Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)

Key features and details

  • Mouse monoclonal [3F9D3D11AF6] to COX6B1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-COX6B1 antibody [3F9D3D11AF6]
    See all COX6B1 primary antibodies

  • Description

    Mouse monoclonal [3F9D3D11AF6] to COX6B1

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human

  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • Human, cow, rat and mouse heart. IF/ICC: HeLa cell line.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

     This product was previously labelled as Cytochrome C Oxidase subunit VIb

     

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)
    Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)

    ICC/IF image of ab110266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)
    Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)
    All lanes : Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human Heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine Heart at 4 µg
    Lane 3 : Isolated mitochondria from Rat Heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse Heart at 10 µg

    Predicted band size: 10 kDa

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