Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24090-74] to SOCS3 - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free
    See all SOCS3 primary antibodies

  • Description

    Rabbit monoclonal [EPR24090-74] to SOCS3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW264.7 treated with 100ng/ml LPS for 4h, Human colon, placenta, Mouse spleen, placenta, HeLa lysates. IHC-P: Human colon, placenta, Mouse spleen and Rat colon. IP: HeLa, RAW 264.7 cells.

  • General notes

    ab280889 is the carrier-free version of ab280884. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280889 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)
    Western blot - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)
    All lanes : Anti-SOCS3 antibody [EPR24090-74] (ab280884) at 1/1000 dilution

    Lane 1 : Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 2 : RAW264.7 treated with 100ng/ml LPS(Lipopolysaccharide) for 4 hours, whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab280884, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:17114447, 32306216, 31238931)

    Exposure time: 26 seconds

     

  • Western blot - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)
    Western blot - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)
    All lanes : Anti-SOCS3 antibody [EPR24090-74] (ab280884) at 1/1000 dilution

    Lane 1 : Human colon tissue lysate
    Lane 2 : Human placenta tissue lysate
    Lane 3 : Human skin tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Mouse placenta tissue lysate
    Lane 6 : Mouse skin tissue lysate
    Lane 7 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 24 kDa
    Observed band size: 23,26 kDa why is the actual band size different from the predicted?



    This data was developed using ab280884, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:17114447, 32306216, 31238931)
    SOCS3 exists in two isoforms of different length that are encoded by one gene
    Negative control: skin (PMID: 22739986)

     Exposure time: 3 minutes

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)

    This data was developed using ab280884, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the stromal cells in human colon. (PMID: 28603075) The section was incubated with ab280884 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)

    This data was developed using ab280884, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human placenta. (PMID: 29201185 ) The section was incubated with ab280884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)

    This data was developed using ab280884, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse spleen. The section was incubated with ab280884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)

    This data was developed using ab280884, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the stromal cells in rat colon. The section was incubated with ab280884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)

    This data was developed using ab280884, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skin tissue labelling SOCS3 with ab280884 at 1/1000 (0.576 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining on human skin. (PMID: 22739986) The section was incubated with ab280884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stain Hematoxylin.


    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)
    Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)

    This data was developed using ab280884, the same antibody clone in a different buffer formulation.

    SOCS3 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug with ab280884 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280884 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2: ab280884 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG1 (ab18443) instead of ab280884 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 58 seconds

     

  • Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)
    Immunoprecipitation - Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)

    This data was developed using ab280884, the same antibody clone in a different buffer formulation.

    SOCS3 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) treated with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours whole cell lysate 10 ug with ab280884 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280884 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) treated with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours whole cell lysate 10 ug

    Lane 2: ab280884 IP in RAW 264.7 treated with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab280884 in treated with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

     

  • Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)
    Anti-SOCS3 antibody [EPR24090-74] - BSA and Azide free (ab280889)

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