Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5719(2)] to NPR-A + NPR-B
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NPR-A + NPR-B antibody [EPR5719(2)] -
Description
Rabbit monoclonal [EPR5719(2)] to NPR-A + NPR-B -
Host species
Rabbit -
Specificity
The immunogen for this product shares 95% homology with NPR-A -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Synthetic peptide within Human NPR-B aa 800-900. The exact sequence is proprietary.
(Peptide available asab150296) -
Positive control
- Human artery, fetal kidney, fetal brain, Mouse brain, Mouse heart, Rat brain, and Rat heart tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5719(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Human artery lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 117 kDa
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All lanes : Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 117 kDa
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All lanes : Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188) at 1/1000 dilution
Lane 1 : GST-tagged recombinant human Natriuretic Peptide Receptor A protein (aa 495 to 1061)
Lane 2 : GST-tagged recombinant human Natriuretic Peptide Receptor B protein (aa 479 to 1047)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 117 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking buffer and concentration: 5% NFDM/TBST
Loading Control: Anti-GST antibody, ab111947
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