Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5026] to Aurora A - Centrosome Marker
  • Suitable for: WB, Flow Cyt, ICC/IF, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Aurora A antibody [EPR5026] - Centrosome Marker
    See all Aurora A primary antibodies

  • Description

    Rabbit monoclonal [EPR5026] to Aurora A - Centrosome Marker

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: BXPC-3, LnCaP, SKBR-3 and HepG2 cell lysates. ICC/IF: HeLa cells.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
    Western blot - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
    All lanes : Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353) at 1/1000 dilution

    Lane 1 : BXPC-3 cell lysate
    Lane 2 : LnCaP cell lysate
    Lane 3 : SKBR-3 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 46 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
    Immunocytochemistry/ Immunofluorescence - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
    Immunofluorescent staining of HeLa cells using ab108353 at 1/100
  • Immunoprecipitation - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
    Immunoprecipitation - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)

    Lane 1: Neuro2a (Mouse neuroblastoma neuroblast) whole cell lysate, 10µg
    Lane 2:     Neuro2a cell lysate 350µg and ab109518 2µg
    Lane 3:     Neuro2a cell lysate, 350µg and rabbit IgG (ab172730) , 2µg

    Purified ab108353 immunoprecipitating Aurora A in Neuro2a cell lysates. Primary antibody was used at a 1/60 dilution (20 µg/ml).  For western blotting, ab108353 at 1/500 and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Blocking and diluting buffer used: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
    Immunoprecipitation - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)

    Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
    Lane 2:     HepG2 cell lysate 350µg and ab109518 2µg
    Lane 3:      HepG2 cell lysate 350µg and rabbit IgG (ab172730) , 2µg

    Purified ab108353 immunoprecipitating Aurora A in Neuro2a cell lysates. Primary antibody was used at a 1/60 dilution (20 µg/ml).  For western blotting, ab108353 at 1/500 and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Blocking and diluting buffer used: 5% NFDM/TBST.

  • Flow Cytometry - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
    Flow Cytometry - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
    Overlay histogram showing HeLa cells stained with ab108353 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108353, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Western blot - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
    Western blot - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
    Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353) at 1/1000 dilution + Neuro-2a cell lysate at 10 µg

    Predicted band size: 46 kDa

  • OI-RD Scanning - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
    OI-RD Scanning - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
    Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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