Antibodies, Proteins, Kits and Reagents for Life Science

324 988 ₸ Product size

100 µl 324 988 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Aurora A antibody [EP1008Y] (ab52973)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1008Y] to Aurora A
Suitable for: WB, IP, IHC-P
Reacts with: Human

Product name

Anti-Aurora A antibody [EP1008Y]
See all Aurora A primary antibodies
Description

Rabbit monoclonal [EP1008Y] to Aurora A
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Aurora A aa 350-450 (C terminal). The exact sequence is proprietary.
Positive control
- HepG2 nuclear cell lysate Human cervical carcinoma tissue IP: HepG2 whole cell lysate.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP1008Y
Isotype

IgG
Research areas

Western blot - Anti-Aurora A antibody [EP1008Y] (ab52973)

Anti-Aurora A antibody [EP1008Y] (ab52973) at 1/50000 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 46 kDa
Observed band size: 46 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora A antibody [EP1008Y] (ab52973)

Formalin-fixed, paraffin-embedded human cervical carcinoma tissue stained for Aurora A with ab52973 (1/100 dilution) in immunohistochemical analysis. Inset panel is a larger magnification of the image.

Inset panel is a larger magnification of the image.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Aurora A antibody [EP1008Y] (ab52973)

Purified ab52973 at 1/50 dilution (2µg) immunoprecipitating Aurora A in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab52973 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52973 in HepG2 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 46 kDa
Anti-Aurora A antibody [EP1008Y] (ab52973)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com