Anti-Aurora A antibody (ab61114)
Key features and details
- Rabbit polyclonal to Aurora A
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Aurora A antibody
See all Aurora A primary antibodies -
Description
Rabbit polyclonal to Aurora A -
Host species
Rabbit -
Specificity
ab61114 detects endogenous levels of total Aurora A protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Synthetic non-phosphopeptide derived from human Aurora A around the phosphorylation site of threonine 288 (R-T-TP-L-C).
-
Positive control
- extracts from 293 cells treated with serum (20%, 15mins).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab61114 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Aurora A antibody (ab61114)All lanes : Anti-Aurora A antibody (ab61114) at 1/500 dilution
Lane 1 : Extracts from 293 cells treated
with serum (20%, 15mins)
Lane 2 : Extracts from 293 cells treated
with serum (20%, 15mins) with immunising Aurora A peptide
Predicted band size: 46 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora A antibody (ab61114)ab61114 staining Aurora A in human ovarian carcinoma.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
