Anti-TNF Receptor I antibody (ab134562)
Key features and details
- Goat polyclonal to TNF Receptor I
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TNF Receptor I antibody
See all TNF Receptor I primary antibodies -
Description
Goat polyclonal to TNF Receptor I -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human TNF Receptor I aa 381-394 (internal sequence) (Cysteine residue).
Sequence:SDHEIDRLELQNGR
Database link: P19438 -
Positive control
- WB: Human Lung lysate, A549, HepG2, K562
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TNF Receptor I antibody (ab134562)All lanes : Anti-TNF Receptor I antibody (ab134562) at 0.5 µg/ml
Lane 1 : A549 (Human lung carcinoma)
Lane 2 : HepG2 (Human liver hepatocellular carcinoma)
Lane 3 : K562 (Human chronic myelogenous leukemia cells from bone marrow)
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
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Western blot - Anti-TNF Receptor I antibody (ab134562)Anti-TNF Receptor I antibody (ab134562) at 0.3 µg/ml + Human Lung lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
