Anti-TNF Receptor I antibody (ab223352)
Key features and details
- Rabbit polyclonal to TNF Receptor I
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TNF Receptor I antibody
See all TNF Receptor I primary antibodies -
Description
Rabbit polyclonal to TNF Receptor I -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Mouse TNF Receptor I aa 1-100.
Database link: P25118 -
Positive control
- WB: Mouse liver lysate. ICC/IF: HeLa (human epithelial cell line from cervix adenocarcinoma) and HaCaT (human keratinocyte cell line) cells. IHC: Mouse back skin.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-TNF Receptor I antibody (ab223352) at 1/1000 dilution + mouse liver lysate at 15 µg
Secondary
Goat anti-rabbit IgG-HRP at 1/2000 dilution
Predicted band size: 50.5 kDaBlocking: 5% skimmed milk in 1X TBST.
Primary incubation: 2 hours at room temperature
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2% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TNF Receptor I (red) using ab223352 at a dilution of 1/100 in ICC/IF (panel B). The secondary antibody is APC Goat Anti-Rabbit used at 1/200 dilution. The nuclear counter stain is DAPI (blue)(panel A). The merged images are shown in panel C.
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2% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TNF Receptor I (green) using ab223352 at a dilution of 1/100 in ICC/IF (panel B). The secondary antibody is FITC Goat Anti-Rabbit used at 1/200 diliution. The nuclear counter stain is DAPI (blue)(panel A) used at 1/40,000 dilution. The merged images are shown in panel C.
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Methanol-fixed HaCaT (human keratinocyte cell line) cells stained for TNF Receptor I (green) using ab223352 at a dilution of 1/100 in ICC/IF. The secondary antibody is FITC Goat Anti-Rabbit at a 1/50 dilution.
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Sections of mouse back skin were stained for TNF Receptor I with ab223352 at a dilution of 1/100 in immunohistochemical analysis.
The secondary antibody was FITC Goat Anti-Rabbit (green) at a 1/50 dilution.