Anti-SIRT1 antibody (ab12193)
Key features and details
- Rabbit polyclonal to SIRT1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SIRT1 antibody
See all SIRT1 primary antibodies -
Description
Rabbit polyclonal to SIRT1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Mouse SIRT1 aa 722-737 (C terminal). With a C-terminal added lysine.
Sequence:ATRQELTDVNYPSDKS
Database link: Q923E4 -
General notes
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody is affinity-purified using the immunogen peptide immobilized on agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SIRT1 antibody (ab12193)All lanes : Anti-SIRT1 antibody (ab12193) at 1 µg/ml
Lane 1 : HeLa Nuclear extract.
Lane 2 : HeLa Nuclear extract. with SIRT1 Immunising Peptide (H. sapens).
Lane 3 : Hct-116 whole cell lysate.
Lane 4 : Hct-116 whole cell lysate. with SIRT1 Immunising Peptide (H. sapens).
Secondary
All lanes : Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate.
Predicted band size: 80 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-SIRT1 antibody (ab12193)Immunocytochemical Immunofluorescence analysis of fixed NIH3T3 cells labelling SIRT1 with ab12193 at a concentration of 20 μg/mL. The secondary used was a Goat Anti-Rabbit IgG, Atto® 488 conjugate. Nuclear staining with DAPI.
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Western blot - Anti-SIRT1 antibody (ab12193)Lane 1 : Anti-SIRT1 antibody (ab12193) at 0.25 µg/ml
Lane 2 : Anti-SIRT1 antibody (ab12193) at 0.5 µg/ml
All lanes : NIH-3T3 Nuclear extract.
Secondary
All lanes : Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate.
Predicted band size: 80 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-SIRT1 antibody (ab12193)Immunocytochemical immunofluorescence analysis of fixed HeLa cells labelling SIRT1 using ab12193 at a concentration of 20 μg/mL. The secondary used was a Goat Anti-Rabbit IgG, Atto® 488 conjugate. Counterstaining was with DAPI against Nuclear DNA.
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Immunocytochemistry/ Immunofluorescence - Anti-SIRT1 antibody (ab12193) Image from Stettner M et al., PLoS One. 2013;8(6):e66079. Fig 5.; doi: 10.1371/journal.pone.0066079. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ab12193 staining SIRT1 in pure rat Schwann cells by ICC/IF (Immunocytochemistry/immunofluorescence) after resveratol (RSV) treatment. Cells were fixed with 4% PFA blocked with 10% Goat serum/ 0.1% Triton x-100/ 0.1% BSA in PBS for 60 minutes at 21°C followed by 10% Goat serum/ 0.5% Triton X-100/ 0.01% BSA in PBS for 15 minutes at 21°C. Samples were incubated with primary antibody (1/100 in PBS + 10% goat serum) overnight at 21°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody. Stimulation of rSCs with RSV led to an increase of SIRT1 expression in densitometry analysis.
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Western blot - Anti-SIRT1 antibody (ab12193) This image is courtesy of an anonymous AbreviewAnti-SIRT1 antibody (ab12193) at 1/500 dilution (for 1 hour at 21°C) + Whole tissue lysate of mouse jejunum and ileum at 10 µg
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 80 kDa
Additional bands at: ~45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Blocking Step: 5 µg/mL milk for 16 hours at 4°C.
