Anti-p63 antibody (ab53039)
Key features and details
- Rabbit polyclonal to p63
- Suitable for: ICC/IF, WB
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-p63 antibody
See all p63 primary antibodies -
Description
Rabbit polyclonal to p63 -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of total p63 protein. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Synthetic peptide derived from human p63. (Peptide available as ab153667.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p63 antibody (ab53039)All lanes : Anti-p63 antibody (ab53039) at 1/500 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract with peptide at 1 µg/ml
Predicted band size: 77 kDa
Observed band size: 77 kDa
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Western blot - Anti-p63 antibody (ab53039)All lanes : Anti-p63 antibody (ab53039) at 1/1000 dilution
Lane 1 : Human p63 full length protein (ab101717) at 0.1 µg
Lane 2 : Human p63 full length protein (ab101717) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 77 kDa
Exposure time: 10 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-p63 antibody (ab53039)Immunofluorescence analysis of A549 (Human lung carcinoma cell line) cells using ab53039 at 1/100 dilution. The picture on the right is blocked with the synthesized peptide.
