Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VPS28 antibody [EPR9880] (ab154793)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9880] to VPS28
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-VPS28 antibody [EPR9880]
    See all VPS28 primary antibodies

  • Description

    Rabbit monoclonal [EPR9880] to VPS28

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human VPS28. The exact sequence is proprietary.
    Database link: Q9UK41

  • Positive control

    • WB: HepG2, Jurkat and LnCaP cell lysates and mouse and rat brain tissue lysates. IP: Jurkat cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
    Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
    Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution (unpurified) + HepG2 cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
    Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
    Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution (purified) + HepG2 cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
    Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
    All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution (unpurified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
    Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
    All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution (purified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
    Western blot - Anti-VPS28 antibody [EPR9880] (ab154793)
    All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1000 dilution (unpurified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : LnCaP cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 25 kDa

  • Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)
    Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)

    ab154793 (unpurified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)
    Immunoprecipitation - Anti-VPS28 antibody [EPR9880] (ab154793)

    ab154793 (purified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-VPS28 antibody [EPR9880] (ab154793)
    Anti-VPS28 antibody [EPR9880] (ab154793)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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