Anti-PIDD1 antibody (ab78389)
Key features and details
- Rabbit polyclonal to PIDD1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PIDD1 antibody
See all PIDD1 primary antibodies -
Description
Rabbit polyclonal to PIDD1 -
Host species
Rabbit -
Specificity
Based on the immunogen information this product will recognise isoforms 1, 2, 3 and 4 of human PIDD1.
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Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human PIDD1 aa 850 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab87817) -
Positive control
- This antibody gave a positive signal in human liver tissue lysate.
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General notes
This product was previously labelled as LRDD
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications MonkeyApplication Abreviews Notes ICC/IF Use a concentration of 1 µg/ml.WB Use a concentration of 1 µg/ml. Detects a band of approximately 99.7 kDa (predicted molecular weight: 99.7 kDa).Notes ICC/IF
Use a concentration of 1 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 99.7 kDa (predicted molecular weight: 99.7 kDa).Target
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Relevance
The death domain (DD) containing protein PIDD is a p53 target gene in an erythroleukemia cell line that undergoes G1 phase arrest and subsequent apoptosis after p53 expression. Independently, PIDD was also described as a DD-containing protein with unknown function. The N-terminal region of PIDD contains seven leucine-rich repeats (LRRs), a protein interaction motif found in various proteins with diverse functions, followed by two ZU-5 domains and a C-terminal DD. PIDD forms a complex with caspase-2 and the adaptor protein RAIDD. Increased PIDD expression results in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli, via interaction with caspase-2 and CRADD/RAIDD. PIDD also promotes apoptosis downstream of p53 as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. PIDD has also been shown to interact with NEMO/IKBKG and RIP1 and enhance sumoylation and ubiquitination of NEMO/IKBKG, an important component for activation of the transcription factor NF-kappa-B. -
Cellular localization
Cytoplasm. Nucleus. -
Database links
- Entrez Gene: 55367 Human
- Omim: 605247 Human
- SwissProt: Q9HB75 Human
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Alternative names
- DKFZp434D229 antibody
- Leucine rich repeats and death domain containing antibody
- Leucine-rich repeats and death domain containing antibody
see all
Images
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Western blot - Anti-PIDD1 antibody (ab78389)Anti-PIDD1 antibody (ab78389) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 99.7 kDa
Observed band size: 99 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa (possible non-specific binding), 37 kDa (possible non-specific binding), 66 kDa (possible isoform)
Exposure time: 3 minutesPIDD1 has seven different isoforms (SwissProt) which all have differing molecular weights. The image shows the primary target (Isoform 1) at 99 kDa as well as isoform 4 (66 kDa).
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Immunocytochemistry/ Immunofluorescence - Anti-PIDD1 antibody (ab78389)ICC/IF image of ab78389 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78389, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HEK293, HepG2 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HepG2, and MCF-7 cells at 1µg/ml.
Protocols
Datasheets and documents
References (3)
ab78389 has been referenced in 3 publications.
- Yao TH et al. Propranolol induces hemangioma endothelial cell apoptosis via a p53-BAX mediated pathway. Mol Med Rep 18:684-694 (2018). PubMed: 29767244
- Klinck R et al. Alternative splicing in osteoclasts and Paget's disease of bone. BMC Med Genet 15:98 (2014). WB, ICC/IF ; Human . PubMed: 25115182
- Lin J et al. The calcium-binding protein S100B down-regulates p53 and apoptosis in malignant melanoma. J Biol Chem 285:27487-98 (2010). WB ; Human . PubMed: 20587415
Images
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Western blot - Anti-PIDD1 antibody (ab78389)Anti-PIDD1 antibody (ab78389) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 99.7 kDa
Observed band size: 99 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa (possible non-specific binding), 37 kDa (possible non-specific binding), 66 kDa (possible isoform)
Exposure time: 3 minutesPIDD1 has seven different isoforms (SwissProt) which all have differing molecular weights. The image shows the primary target (Isoform 1) at 99 kDa as well as isoform 4 (66 kDa).
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Immunocytochemistry/ Immunofluorescence - Anti-PIDD1 antibody (ab78389)ICC/IF image of ab78389 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78389, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HEK293, HepG2 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HepG2, and MCF-7 cells at 1µg/ml.
