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Cancer Oncoproteins/suppressors Tumor suppressors p53 pathway

Anti-SIRT1 (phospho T530) antibody [EPNCIR153] - BSA and Azide free (ab249273)

Anti-SIRT1 (phospho T530) antibody [EPNCIR153] - BSA and Azide free (ab249273)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCIR153] to SIRT1 (phospho T530) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-SIRT1 (phospho T530) antibody [EPNCIR153] - BSA and Azide free
    See all SIRT1 primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR153] to SIRT1 (phospho T530) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody only detects SIRT1 when phosphorylated at threonine 530.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human SIRT1 (phospho T530). The exact sequence is proprietary.

  • General notes

    ab249273 is the carrier-free version of ab156585 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249273 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Mirit Aladjem. Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPNCIR153
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • p53 Pathway
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Other Nuclear Bodies
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class III / Sir2 class
    • Metabolism
    • Types of disease
    • Obesity

Images

  • Western blot - Anti-SIRT1 (phospho T530) antibody [EPNCIR153] - BSA and Azide free (ab249273)
    Western blot - Anti-SIRT1 (phospho T530) antibody [EPNCIR153] - BSA and Azide free (ab249273)
    All lanes : Anti-SIRT1 (phospho T530) antibody [EPNCIR153] (ab156585) at 1/1000 dilution

    Lane 1 : 293T cell lysates
    Lane 2 : 293T cell lysates treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 81 kDa



    This data was developed using ab156585, the same antibody clone in a different buffer formulation.

     

  • Anti-SIRT1 (phospho T530) antibody [EPNCIR153] - BSA and Azide free (ab249273)
    Anti-SIRT1 (phospho T530) antibody [EPNCIR153] - BSA and Azide free (ab249273)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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