Anti-TIGAR antibody [M2 - P4H2] (ab64622)
![Anti-TIGAR antibody [M2 - P4H2] (ab64622)](https://www.abcam.com/ps/products/64/ab64622/Images/ab64622-77999-anti-tigar-antibody-m2-p4h2-western-blot.jpg "Anti-TIGAR antibody [M2 - P4H2] (ab64622)")
Key features and details
- Mouse monoclonal [M2 - P4H2] to TIGAR
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TIGAR antibody [M2 - P4H2]
See all TIGAR primary antibodies -
Description
Mouse monoclonal [M2 - P4H2] to TIGAR -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human TIGAR aa 200-300.
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Positive control
- Recombinant Human TIGAR protein (ab115509) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: U20S cell line Jurkat cell line
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
M2 - P4H2 -
Myeloma
Ag 8563 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-TIGAR antibody [M2 - P4H2] (ab64622)All lanes : Anti-TIGAR antibody [M2 - P4H2] (ab64622) at 10 µg/ml
Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 120 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
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![Immunocytochemistry/ Immunofluorescence - Anti-TIGAR antibody [M2 - P4H2] (ab64622)](https://www.abcam.com/ps/products/64/ab64622/Images/ab64622-77998-anti-tigar-antibody-m2-p4h2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TIGAR antibody [M2 - P4H2] (ab64622)ICC/IF image of ab64622 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64622, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HepG2 and MCF7 cells fixed in 4% PFA at 1ug/ml. -
![Flow Cytometry - Anti-TIGAR antibody [M2 - P4H2] (ab64622)](https://www.abcam.com/ps/products/64/ab64622/Images/ab64622-77997-anti-tigar-antibody-m2-p4h2-flow-cytometry.jpg)
Flow Cytometry - Anti-TIGAR antibody [M2 - P4H2] (ab64622)Overlay histogram showing HeLa cells stained with ab64622 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab64622, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
