Anti-Bovine Serum Albumin antibody [5F9] (ab9092)
![Anti-Bovine Serum Albumin antibody [5F9] (ab9092)](https://www.abcam.com/ps/products/9/ab9092/Images/ab9092_1.gif "Anti-Bovine Serum Albumin antibody [5F9] (ab9092)")
Key features and details
- Mouse monoclonal [5F9] to Bovine Serum Albumin
- Suitable for: WB
- Reacts with: Cow
- Isotype: IgG1
Overview
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Product name
Anti-Bovine Serum Albumin antibody [5F9]
See all Bovine Serum Albumin primary antibodies -
Description
Mouse monoclonal [5F9] to Bovine Serum Albumin -
Host species
Mouse -
Specificity
This antibody recognizes a 66 kDa band corresponding to bovine serum albumin monomer. No cross reactivity is detected against other serum proteins by immunoblot. No reactivity is detected by ELISA against human serum albumin or ovalbumin. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Cow
Does not react with: Chicken, Human
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5F9 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
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Western blot - Anti-Bovine Serum Albumin antibody [5F9] (ab9092)
Anti-Bovine Serum Albumin is shown to detect BSA in bovine serum diluted 1:100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2). In both lanes 20 ul of sample was applied. Primary antibody is diluted 1:5000 in PBS and is reacted for 1.5 h at room temperature.
Detection occurs using a 1:5000 dilution of IRDye800TM Conjugated Goat-a-Mouse IgG reacted for 1 h at room temperature. Visualization is by near-infrared (IR) western blotting analysis using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR Biosciences, Lincoln, NE.
Anti-Bovine Serum Albumin is shown to detect BSA in bovine serum diluted 1:100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2). In both lanes 20 ul of sample was applied. Primary antibody is diluted 1:5000 in PBS and is reacted for 1.5 h at room temperature.
Detection occurs using a 1:5000 dilution of IRDye800
