Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12774] to Bovine Serum Albumin
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Cow, Human

Overview

  • Product name

    Anti-Bovine Serum Albumin antibody [EPR12774]
    See all Bovine Serum Albumin primary antibodies

  • Description

    Rabbit monoclonal [EPR12774] to Bovine Serum Albumin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Cow
    WB
    Mouse
    Cow
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, Human plasma, human serum, HeLa whole cell lysate (ab150035), bovine serum albumin, mouse kidney and spleen, rat kidney, spleen and brain, Raw 264.7, PC12 cell lysates, Human liver tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12774

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Bovine liver labeling Serum albumin with ab192603 at 1/300 dilution.  A prediluted HRP Polymer Rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    All lanes : Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) at 1/1000 dilution

    Lane 1 : Mouse kidney
    Lane 2 : Mouse spleen
    Lane 3 : Rat brain
    Lane 4 : Rat kidney
    Lane 5 : Rat spleen
    Lane 6 : Raw264.7 cell lysate
    Lane 7 : PC-12 cell lysate
    Lane 8 : NIH-3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

  • Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) at 1/1000 dilution + Bovine Serum albumin at 0.001 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

  • Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) at 1/10000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

  • Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    All lanes : Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Human plasma
    Lane 3 : Human serum

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

  • Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)

© 2021 Astana Biomed Group LLP. All rights reserved.