Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GPNMB antibody [SP299] (ab227695)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP299] to GPNMB
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-GPNMB antibody [SP299]
    See all GPNMB primary antibodies

  • Description

    Rabbit monoclonal [SP299] to GPNMB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human GPNMB aa 1-500. The exact sequence is proprietary.
    Database link: Q14956

  • Positive control

    • WB: A-375 cell lysate. Flow Cyt: A-375 cells. IHC-P: Human tonsil, reactive lymph node, lung, colon, lung squamous cell carcinoma and cervical squamous cell carcinoma tissues.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS by protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    SP299

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GPNMB antibody [SP299] (ab227695)
    Western blot - Anti-GPNMB antibody [SP299] (ab227695)
    Anti-GPNMB antibody [SP299] (ab227695) at 1/100 dilution + A-375 (human malignant melanoma cell line) whole cell lysate

    Predicted band size: 64 kDa

  • Flow Cytometry - Anti-GPNMB antibody [SP299] (ab227695)
    Flow Cytometry - Anti-GPNMB antibody [SP299] (ab227695)

    Flow Cytometry analysis of A-375 (human malignant melanoma cell line) cells, labeling GPNMB with ab227695 at 1/100 dilution (green) compared to a Rabbit IgG negative control (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)

    Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)

    Formalin-fixed, paraffin-embedded human lung tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)

    Formalin-fixed, paraffin-embedded human reactive lymph node tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)

    Formalin-fixed, paraffin-embedded human colon tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Anti-GPNMB antibody [SP299] (ab227695)
    Anti-GPNMB antibody [SP299] (ab227695)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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