Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸ 10 µl 117 264 ₸

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Anti-GPNMB antibody [EPR18226-147] (ab188222)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18226-147] to GPNMB
Suitable for: WB, ICC/IF, Flow Cyt, IP
Reacts with: Mouse

Product name

Anti-GPNMB antibody [EPR18226-147]
See all GPNMB primary antibodies
Description

Rabbit monoclonal [EPR18226-147] to GPNMB
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: L-929 and B16-F0 whole cell lysates. ICC/IF: L-929 cells. Flow Cyt: L-929 cells. IP: L-929 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18226-147
Isotype

IgG
Research areas

Western blot - Anti-GPNMB antibody [EPR18226-147] (ab188222)

All lanes : Anti-GPNMB antibody [EPR18226-147] (ab188222) at 1/5000 dilution

Lane 1 : L-929 (mouse connective tissue fibroblast cell line) whole cell lysate
Lane 2 : B16-F0 (mouse melanoma epithelial cell-like cell line) whole cell lysate
Lane 3 : EL4 (mouse lymphoma T lymphocyte cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 64 kDa
Observed band size: 120,65,95 kDa
why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular mass observed is consistent with what has been described in the literature (PMID: 19320736).

Negative control: EL4 (PMID: 19320736).
Immunoprecipitation - Anti-GPNMB antibody [EPR18226-147] (ab188222)

GPNMB was immunoprecipitated from 0.35 mg of L-929 (mouse connective tissue fibroblast cell line) whole cell lysate with ab188222 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188222 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: L-929 whole cell lysate 10 µg (Input).

Lane 2: ab188222 IP in L-929 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188222 in L-929 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 minute.
Flow Cytometry - Anti-GPNMB antibody [EPR18226-147] (ab188222)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized EL4 (mouse lymphoma T lymphocyte cell line; left panel) and L-929 (mouse connective tissue fibroblast cell line; right panel) cell lines labeling GPNMB with ab188222 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control: EL4 (PMID: 19320736)(left panel).
Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [EPR18226-147] (ab188222)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized L-929 (mouse connective tissue fibroblast cell line) and EL4 (mouse lymphoma T lymphocyte cell line) cells labeling GPNMB with ab188222 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining in L-929 cells.

Negative control: EL4 (PMID:19320736).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Anti-GPNMB antibody [EPR18226-147] (ab188222)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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