Human Surfactant protein D / SP-D Antibody Pair - BSA and Azide free (ab244174)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human Surfactant protein D / SP-D Antibody Pair - BSA and Azide free
See all Surfactant protein D/SP-D kits -
Assay type
ELISA set -
Range
125 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human SP-D. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab239431), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Surfactant protein D / SP-D Capture Antibody (unconjugated) 1 x 100µg Human Surfactant protein D / SP-D Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. -
Sequence similarities
Belongs to the SFTPD family.
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain. -
Post-translational
modificationsThe N-terminus is blocked.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. -
Cellular localization
Secreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film. - Information by UniProt
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Alternative names
- COLEC 7
- COLEC7
- Collectin-7
see all -
Database links
- Entrez Gene: 6441 Human
- Omim: 178635 Human
- SwissProt: P35247 Human
- Unigene: 253495 Human
Images
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Sandwich ELISA - Human Surfactant protein D / SP-D Antibody Pair - BSA and Azide free (ab244174)To learn more about the advantages of recombinant antibodies see here.
