Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 16, 2021

Anti-GPNMB antibody [EPR22011-11] (ab222109)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22011-11] to GPNMB
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-GPNMB antibody [EPR22011-11]
See all GPNMB primary antibodies
Description

Rabbit monoclonal [EPR22011-11] to GPNMB
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: SK-MEL-28 whole cell lysate. IHC-P: Human melanoma and colonic carcinoma tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22011-11
Isotype

IgG
Research areas

Western blot - Anti-GPNMB antibody [EPR22011-11] (ab222109)

All lanes : Anti-GPNMB antibody [EPR22011-11] (ab222109) at 1/1000 dilution

Lanes 1-2 : SK-MEL-28 (human malignant melanoma) whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 64 kDa
Observed band size: 120,95 kDa
why is the actual band size different from the predicted?

Exposure time: 81 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular mass observed is consistent with what has been described in the literature (PMID: 19320736).

Negative control: HeLa (PMID: 20056711)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [EPR22011-11] (ab222109)

Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling GPNMB with ab222109 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in tumor cells of human melanoma (PMID: 19320736). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [EPR22011-11] (ab222109)

Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue labeling GPNMB with ab222109 at 1/1000 dilution, followed by aGoat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on stroma cells of human colonic carcinoma (PMID: 20215530). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-GPNMB antibody [EPR22011-11] (ab222109)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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