Anti-TRAP/CD40L antibody [CD40LG/2761] (ab257319)
![Anti-TRAP/CD40L antibody [CD40LG/2761] (ab257319)](https://www.abcam.com/ps/products/257/ab257319/Images/ab257319-352743-anti-trapcd40l-antibody-cd40lg-2761-immunohistochemistry-spleen-human.jpg "Anti-TRAP/CD40L antibody [CD40LG/2761] (ab257319)")
Key features and details
- Mouse monoclonal [CD40LG/2761] to TRAP/CD40L
- Suitable for: IHC-P, Flow Cyt, ICC/IF, SDS-PAGE
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-TRAP/CD40L antibody [CD40LG/2761]
See all TRAP/CD40L primary antibodies -
Description
Mouse monoclonal [CD40LG/2761] to TRAP/CD40L -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P Human
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Immunogen
Recombinant fragment within Human TRAP/CD40L aa 108-261. The exact sequence is proprietary.
Database link: P29965 -
Positive control
- IHC-P: Human spleen and tonsil tissue; Flow Cyt: Jurkat cells; ICC/IF: Jurkat cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
CD40LG/2761 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP/CD40L antibody [CD40LG/2761] (ab257319)
Immunohistochemical analysis of fomalin-fixed, paraffin-embedded human spleen tissue staining TRAP/CD40L with ab257319 at 2µg/ml.
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![Immunocytochemistry/ Immunofluorescence - Anti-TRAP/CD40L antibody [CD40LG/2761] (ab257319)](https://www.abcam.com/ps/products/257/ab257319/Images/ab257319-352740-anti-trap-cd40l-antibody-cd40lg-2761-immunofluorescence-jurkat-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TRAP/CD40L antibody [CD40LG/2761] (ab257319)Immunocytochemical/Immunofluorescent analysis of paraformaldehyde-fixed Jurkat cells staining TRAP/CD40L with ab257319 at 2µg/ml, followed by a goat anti-mouse conjugated to CF488. Nuclei are stained with Reddot.
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![Flow Cytometry - Anti-TRAP/CD40L antibody [CD40LG/2761] (ab257319)](https://www.abcam.com/ps/products/257/ab257319/Images/ab257319-352739-anti-trap-cd40l-antibody-cd40lg-2761-flow-cytometry-jurkat-human.jpg)
Flow Cytometry - Anti-TRAP/CD40L antibody [CD40LG/2761] (ab257319)Flow Cytometric analysis of Jurkat cells staining TRAP/CD40L with ab257319 at 2µg/million cells, followed by a Goat anti-Mouse secondary antibody = blue. Isotype control = red.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP/CD40L antibody [CD40LG/2761] (ab257319)](https://www.abcam.com/ps/products/257/ab257319/Images/ab257319-352742-anti-trap-cd40l-antibody-cd40lg-2761-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP/CD40L antibody [CD40LG/2761] (ab257319)Immunohistochemical analysis of fomalin-fixed, paraffin-embedded human tonsil tissue staining TRAP/CD40L with ab257319 at 2µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP/CD40L antibody [CD40LG/2761] (ab257319)](https://www.abcam.com/ps/products/257/ab257319/Images/ab257319-352741-anti-trap-cd40l-antibody-cd40lg-2761-immunohistochemistry-spleen-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP/CD40L antibody [CD40LG/2761] (ab257319)Immunohistochemical analysis of fomalin-fixed, paraffin-embedded human spleen tissue staining TRAP/CD40L with ab257319 at 2µg/ml.
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![SDS-PAGE - Anti-TRAP/CD40L antibody [CD40LG/2761] (ab257319)](https://www.abcam.com/ps/products/257/ab257319/Images/ab257319-352736-anti-trapcd40l-antibody-cd40lg-2761-sds-page.jpg)
SDS-PAGE - Anti-TRAP/CD40L antibody [CD40LG/2761] (ab257319)SDS-PAGE analysis of ab257319 under non-reducing (lane 1) and reducing conditions (lane 2).
