Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [OTI4A11] to SSX2
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human, African green monkey
Isotype: IgG1

Product name

Anti-SSX2 antibody [OTI4A11]
See all SSX2 primary antibodies
Description

Mouse monoclonal [OTI4A11] to SSX2
Host species

Mouse
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Human, African green monkey
Immunogen

Recombinant full length protein corresponding to Human SSX2. (Expressed in HEK-293T cells).
Database link: Q16385
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY SSX2 cDNA. IHC-P: Human breast adenocarcinoma, colon, colon adenocarcinoma, ovary, ovary adenocarcinoma, endometrium, prostate and bladder carcinoma tissue. ICC/IF: COS7 cells transiently transfected with pCMV6-ENTRY SSX2.
General notes

Clone OTI4A11 (formerly 4A11).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

OTI4A11
Isotype

IgG1
Research areas

Western blot - Anti-SSX2 antibody [OTI4A11] (ab236415)

All lanes : Anti-SSX2 antibody [OTI4A11] (ab236415) at 1/2000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY SSX2 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 22 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)

Paraffin-embedded human breast adenocarcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)

Paraffin-embedded human colon tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)

Paraffin-embedded human colon adenocarcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)

Paraffin-embedded human ovary tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)

Paraffin-embedded human ovary adenocarcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)

Paraffin-embedded human endometrium tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)

Paraffin-embedded human prostate tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2 antibody [OTI4A11] (ab236415)

Paraffin-embedded human bladder carcinoma tissue stained for SSX2 using ab236415 at 1/50 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-SSX2 antibody [OTI4A11] (ab236415)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY SSX2 stained for SSX2 (green) using ab236415 at 1/100 dilution in ICC/IF.

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