Anti-CD4 antibody [Campath-9H] - Chimeric (ab275975)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Campath-9H] to CD4 - Chimeric
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD4 antibody [Campath-9H] - Chimeric
See all CD4 primary antibodies -
Description
Rabbit monoclonal [Campath-9H] to CD4 - Chimeric -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human -
Immunogen
Tissue, cells or virus corresponding to Human CD4. Rat T-cell line (NB2) transfected with the human CD4.
Database link: P01730 -
Positive control
- Flow cyt: Human lymphocytes.
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General notes
This chimeric rabbit antibody was made using the variable domain sequences of the original rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Campath-9H -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Flow cytometry of human lymphocytes labeling CD4 with ab275975 (panel B) compared with an an isotype control (panel A) at 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073) and cells analysed on a FACSCanto flow-cytometer.
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