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Immunology Adaptive Immunity T Cells CD

Anti-CD1d antibody [EPR22526-72] (ab229903)

Price and availability

307 566 ₸

Availability

Order now and get it on Friday March 19, 2021

Anti-CD1d antibody [EPR22526-72] (ab229903)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22526-72] to CD1d
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-CD1d antibody [EPR22526-72]
    See all CD1d primary antibodies
  • Description

    Rabbit monoclonal [EPR22526-72] to CD1d
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human lymph node and thymus lysates; Jurkat and MOLT-4 whole cell lysates. IP: Jurkat whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22526-72
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Immunoglobulins
    • Receptors
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Mast Cell Lineage

Images

  • Western blot - Anti-CD1d antibody [EPR22526-72] (ab229903)
    Western blot - Anti-CD1d antibody [EPR22526-72] (ab229903)
    All lanes : Anti-CD1d antibody [EPR22526-72] (ab229903) at 1/1000 dilution

    Lane 1 : Human lymph node lysate
    Lane 2 : Human thymus lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
    Lane 5 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 6 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?



    Exposure time: Lane 1: 3 minutes; Lanes 2-6: 48 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 10809957).

    Negative control: SH-SY5Y (PMID: 26749374), K562 (PMID: 21037579).

  • Immunoprecipitation - Anti-CD1d antibody [EPR22526-72] (ab229903)
    Immunoprecipitation - Anti-CD1d antibody [EPR22526-72] (ab229903)

    CD1d was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole lysate with ab229903 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229903 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: Jurkat whole cell lysate 10 µg (Input). 

    Lane 2: ab229903 IP in Jurkat whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229903 in Jurkat whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-CD1d antibody [EPR22526-72] (ab229903)
    Anti-CD1d antibody [EPR22526-72] (ab229903)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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