Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT1 antibody [15H3] (ab155933)

Key features and details

  • Mouse monoclonal [15H3] to STAT1
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Mouse, Human, Non human primates, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-STAT1 antibody [15H3]
    See all STAT1 primary antibodies

  • Description

    Mouse monoclonal [15H3] to STAT1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Mouse
    Human
    African green monkey
    See all applications and species data

  • Immunogen

    Protein expressed in 293T cells transfected with Human STAT1 expression vector.

  • Positive control

    • A2058, HeLa and MCF7 cells; A549, 293T, Jurkat, A431, U2OS, COS7 whole cell lysates.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-STAT1 antibody [15H3] (ab155933)
    Western blot - Anti-STAT1 antibody [15H3] (ab155933)
    All lanes : Anti-STAT1 antibody [15H3] (ab155933) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : A431 whole cell lysate
    Lane 5 : U2OS whole cell lysate
    Lane 6 : COS7 whole cell lysate
    Lane 7 : 3T3L1 whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat anti-mouse HRP conjugated antibody at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 87 kDa



    SDS PAGE: 4-20% Tris-HCl polyacrylamide gel.
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [15H3] (ab155933)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [15H3] (ab155933)
    Immunofluorescent analysis of formaldehyde-fixed A2058 cells labeling STAT1 with ab155933 at 1/20 dilution (green, left image compared with control cells (right image) followed by a DyLight 488-conjugated secondary. Actin stained with Phalloidin (red) and nuclei stained with DAPI (blue).
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [15H3] (ab155933)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [15H3] (ab155933)
    Immunofluorescent analysis of formaldehyde-fixed HeLa cells labeling STAT1 with ab155933 at 1/20 dilution (green, left image compared with control cells (right image) followed by a DyLight 488-conjugated secondary. Actin stained with Phalloidin (red) and nuclei stained with DAPI (blue).
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [15H3] (ab155933)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [15H3] (ab155933)
    Immunofluorescent analysis of formaldehyde-fixed MCF7 cells labeling STAT1 with ab155933 at 1/20 dilution (green, left image compared with control cells (right image) followed by a DyLight 488-conjugated secondary. Actin stained with Phalloidin (red) and nuclei stained with DAPI (blue).
  • Immunoprecipitation - Anti-STAT1 antibody [15H3] (ab155933)
    Immunoprecipitation - Anti-STAT1 antibody [15H3] (ab155933)
    Immunoprecipitation analysis of STAT1 in U2OS cells.
    750 µg of U2OS cell lysate immunoprecipitated with 2 µg ab155933.
    All lanes: ab155933 at 1/1000 dilution.
    Lane 1: U2OS whole cell lysate.
    Lane 2: U2OS whole cell lysate immunopreciptated with ab155933.
    Western Blot: 4-20% Tris-HCl polyacrylamide gel.
    Secondary: Goat anti-mouse HRP conjugated antibody at 1/20000 dilution.
    Predicted band size : 87 kDa.

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