Antibodies, Proteins, Kits and Reagents for Life Science

308 236 ₸ Product size

100 µg 308 236 ₸

Order now and get it on Thursday February 25, 2021

Anti-STAT3 (phospho S727) antibody (ab30647)

Key features and details

Rabbit polyclonal to STAT3 (phospho S727)
Suitable for: IHC-P, WB
Reacts with: Human, African green monkey
Isotype: IgG

Product name

Anti-STAT3 (phospho S727) antibody
See all STAT3 primary antibodies
Description

Rabbit polyclonal to STAT3 (phospho S727)
Host species

Rabbit
Specificity

ab30647 detects endogenous levels of STAT3 only when phosphorylated at Serine 727.
Tested applications

Suitable for: IHC-P, WBmore details
Species reactivity

Reacts with: Human, African green monkey
Predicted to work with: Mouse
Immunogen

Synthetic peptide corresponding to Human STAT3 (phospho S727). The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of serine 727 (P-M-SP-P-R).
Database link: P40763
(Peptide available as ab43618, ab82053)

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride

PBS without Mg2+ and Ca2+
Concentration information loading...
Purity

Protein A purified
Purification notes

ab30647 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality

Polyclonal
Isotype

IgG
Research areas

Western blot - Anti-STAT3 (phospho S727) antibody (ab30647)

All lanes : Anti-STAT3 (phospho S727) antibody (ab30647)

Lane 1 : HeLa cells extract
Lane 2 : HeLa cells extract with Synthesized non-phosphopeptide
Lane 3 : HeLa cells extract with Synthesized phosphopeptide

Predicted band size: 88 kDa
Observed band size: 88 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody (ab30647)

ab30647 at 1/50 dilution staining STAT3 (phospho S727) in human breast carcinoma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). The left image show staining by ab30647 without specific peptide while right image show staining after blocking with peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody (ab30647)

Immunohistochemical analysis of human uterus tissue stained with ab30647 at 1/200 dilution at 4^oC overnight. The negative control is stained with secondary antibody only.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody (ab30647)

Immunohistochemical analysis of human lung tissue stained with ab30647 at 1/200 dilution at 4^oC overnight. The negative control is stained with secondary antibody only.
Western blot - Anti-STAT3 (phospho S727) antibody (ab30647)

All lanes : Anti-STAT3 (phospho S727) antibody (ab30647) at 1/500 dilution

Lane 1 : COS7 cell lysate
Lane 2 : COS7 cell lysate with phospho peptide

Predicted band size: 88 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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