Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2147Y] to STAT3 (phospho Y705) - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, IP, IHC-P, WB, Dot blot
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free
    See all STAT3 primary antibodies

  • Description

    Rabbit monoclonal [EP2147Y] to STAT3 (phospho Y705) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysate treated with alpha-interferon. IHC-P: Human colon carcinoma, kidney and thyroid carcinoma tissues and mouse spleen tissue ICC/IF: HeLa cells treated with alpha-interferon; U251 cells. IP: A431 cells treated with EGF. Flow Cyt: A431 cells.

  • General notes

    Ab171358 is the carrier-free version of ab76315. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab171358 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labeling STAT3 (phospho Y705) with purified ab76315 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76315).

  • Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)

    This ICC/IF data was generated using the same anti-phospho STAT3 Y705 antibody clone, EP2147Y, in a different buffer formulation (cat# ab76315).

    Immunocytochemistry/Immunofluorescence analysis of HeLa +/- IFN-α (50ng/mL, 5 minutes) cells labelling STAT3 (phospho Y705) with ab76315 at 1/500 (4.3 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1500) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.

  • Immunoprecipitation - Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)
    Immunoprecipitation - Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)

    ab76315 (purified) at 1/30 immunoprecipitating STAT3 (phospho Y705) in A431 (Human epidermoid carcinoma cell line) cell lysate treated with EGF. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking/Dilution buffer: 5% NFDM/TBST.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76315).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)

    This IHC data was generated using the same anti-phospho STAT3 Y705 antibody clone, EP2147Y, in a different buffer formulation (cat# ab76315).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling STAT3 (phospho Y705) with unpurified ab76315 at 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)

    Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue using  untreated (left) or alkaline phosphatase-treated (right) labeling STAT3 (phospho Y705) with ab76315 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H& L (HRP) (ab97051) at 1/500 dilution.

    Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76315).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)
    Flow Cytometry - Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)

    This data was developed using ab76315, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/mL EGF for 10min (Red) / Untreated control (Green) cells labelling STAT3 with ab76315 at 1/500 dilution (0.1µg) (Red) and Green compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
  • Dot Blot - Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)
    Dot Blot - Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)

    Dot blot analysis of STAT3 single phospho peptide pY705 (lane 1) and STAT3 non-phospho peptide (lane 2) with ab76315 at 1/1000. Blocking and dilution buffer was 5% NFDM/TBST. The secondary antibody used was ab97051 peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/100,000.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76315).

  • Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)
    Anti-STAT3 (phospho Y705) antibody [EP2147Y] - BSA and Azide free (ab171358)

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