Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15961] to NDUFB8 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC, IP, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free
    See all NDUFB8 primary antibodies

  • Description

    Rabbit monoclonal [EPR15961] to NDUFB8 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Rat
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251160 is the carrier-free version of ab192878. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251160 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    Western blot - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    All lanes : Anti-NDUFB8 antibody [EPR15961] (ab192878) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : NDUFB8 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 22 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab192878, the same antibody clone in a different buffer formulation.

    Lanes 1 - 2 Merged signal (red and green). Green - ab192878 observed at 19 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab192878 was shown to specifically react with NDUFB8 in wild-type HAP1 cells as signal was lost in NDUFB8 knockout cells. Wild-type and NDUFB8 knockout samples were subjected to SDS-PAGE. ab192878 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)

    This data was developed using ab192878, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB8 with ab192878 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    Flow Cytometry - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)

    This data was developed using ab192878, the same antibody clone in a different buffer formulation.

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling NDUFB8 antibody (red) with purified ab192878 at a dilution of 1/30. Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/2000. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
  • Western blot - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    Western blot - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    All lanes : Anti-NDUFB8 antibody [EPR15961] (ab192878) at 1/5000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human tonsil lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 19 kDa why is the actual band size different from the predicted?



    This data was developed using ab192878, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    Western blot - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    Anti-NDUFB8 antibody [EPR15961] (ab192878) at 1/20000 dilution + Human fetal heart lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 19 kDa why is the actual band size different from the predicted?



    This data was developed using ab192878, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    Western blot - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    All lanes : Anti-NDUFB8 antibody [EPR15961] (ab192878) at 1/5000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 19 kDa why is the actual band size different from the predicted?



    This data was developed using ab192878, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    This data was developed using ab192878, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling NDUFB8 with ab192878 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    This data was developed using ab192878, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling NDUFB8 with ab192878 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    Immunoprecipitation - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    This data was developed using ab192878, the same antibody clone in a different buffer formulation.Western blot analysis of NDUFB8 immunoprecipitated from Human fetal heart lysate using ab192878 at 1/20 dilution. Lane 1: Human fetal liver lysate. Lane 2: PBS instead of Human fetal liver lysate.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.Blocking/Dilution buffer: 5% NFDM/TBST.
  • Immunocytochemistry - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    Immunocytochemistry - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    This data was developed using ab192878, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling NDUFB8 with ab192878 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary antibody at 1/400 dilution. Nuclear counter stained is DAPI (blue).
  • Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)
    Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (ab251160)

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