Anti-NDUFB8 antibody (ab134367)
Key features and details
- Rabbit polyclonal to NDUFB8
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NDUFB8 antibody
See all NDUFB8 primary antibodies -
Description
Rabbit polyclonal to NDUFB8 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human NDUFB8 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab156057) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Heart; Human Heart Mitonchondrial; Mouse Heart; Human Skeletal Muscle; Mouse Skeletal Muscle. This antibody gave a positive result in IHC in the following FFPE tissue: Human Heart muscle.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 21 kDa). Target
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Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
Sequence similarities
Belongs to the complex I NDUFB8 subunit family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4714 Human
- Entrez Gene: 67264 Mouse
- Entrez Gene: 293991 Rat
- Omim: 602140 Human
- SwissProt: O95169 Human
- SwissProt: Q9D6J5 Mouse
- Unigene: 523215 Human
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Alternative names
- ASHI antibody
- CI-ASHI antibody
- Complex I ASHI subunit antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody (ab134367)
IHC image of NDUFB8 staining in Human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134367, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-NDUFB8 antibody (ab134367)All lanes : Anti-NDUFB8 antibody (ab134367) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human Heart Mitochondrial Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 5 : Skeletal Muscle (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
The band observed at 19 kDa could potentially be a cleaved form of NDUFB8 due to the presence of a 28 amino acid transit peptide. This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab134367 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
References (0)
ab134367 has not yet been referenced specifically in any publications.
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody (ab134367)
IHC image of NDUFB8 staining in Human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134367, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-NDUFB8 antibody (ab134367)All lanes : Anti-NDUFB8 antibody (ab134367) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human Heart Mitochondrial Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 5 : Skeletal Muscle (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
The band observed at 19 kDa could potentially be a cleaved form of NDUFB8 due to the presence of a 28 amino acid transit peptide. This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab134367 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
