All lanes : Anti-NDUFB8 antibody [20E9DH10C12] (ab110242) at 0.5 µg/ml

Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from cow heart at 1 µg
Lane 3 : Isolated mitochondria from rat heart at 10 µg
Lane 4 : Isolated mitochondria from mouse heart at 10 µg

Predicted band size: 22 kDa



Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

Immunohistochemistry in serial sections of the muscle of patient AIV-5

The patient is sufferening from Spastic paraplegia 7.

Immunohistochemistry for complex I (NDUFB8) (A), complex II (B), complex III (C) and COX/SDH histochemistry (D) in serial sections of the muscle of patient AIV-5. There are complex I, III and IV deficient fibres, but complex I deficiency is most pronounced. Arrows mark serial sections of the same muscle fibers stained for different complexes.

NDUFB8 (also refered to as complex I) was detected using ab110242 at 1/100 dilution).

(After Figure 3 of Wedding et al)

Skeletal muscle immunohistochemistry using ab110242 on frozen tissue sections from a patient with a single large deletion of the mtDNA show a mosaic of complex I positive and complex I negative fibers.