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Signal Transduction Signaling Pathway Nuclear Signaling STATs

Anti-STAT3 (phospho S727) antibody (ab131103)

Price and availability

291 484 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-STAT3 (phospho S727) antibody (ab131103)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to STAT3 (phospho S727)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-STAT3 (phospho S727) antibody
    See all STAT3 primary antibodies
  • Description

    Rabbit polyclonal to STAT3 (phospho S727)
  • Host species

    Rabbit
  • Specificity

    ab131103 detects endogenous levels of STAT3 only when phosphorylated at serine 727.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human STAT3 (phospho S727) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    P-M-S(p)-P-R


    Database link: P40763
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa and HepG2 cell extract treated with PMA; HeLa cells; Human breast carcinoma tissue
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131103 was purified by affinity-chromatography using epitope-specific phosphopetide. Non-phospho specific antibodies were removed by chromatography using non-phosphopetide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • STAT family
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Transcription factors
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules

Images

  • Western blot - Anti-STAT3 (phospho S727) antibody (ab131103)
    Western blot - Anti-STAT3 (phospho S727) antibody (ab131103)
    All lanes : Anti-STAT3 (phospho S727) antibody (ab131103) at 1/500 dilution

    Lane 1 : HeLa cell extract untreated
    Lane 2 : HeLa cell extract treated with PMA
    Lane 3 : HepG2 cell extract untreated
    Lane 4 : HepG2 cell extract treated with PMA

    Predicted band size: 88 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody (ab131103)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody (ab131103)
    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling STAT3 (phospho S727) with ab131103 at 1/50 dilution. Right panel was preincubated with blocking peptide.
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho S727) antibody (ab131103)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho S727) antibody (ab131103)
    Immunofluorescence analysis of methanol-fixed HeLa cells labelling STAT3 (phospho S727) with ab131103 at 1/100 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-STAT3 (phospho S727) antibody (ab131103)

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