Anti-RAIN antibody (ab129820)
Key features and details
- Rabbit polyclonal to RAIN
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-RAIN antibody
See all RAIN primary antibodies -
Description
Rabbit polyclonal to RAIN -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
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Immunogen
corresponding to RAIN.
(Peptide available asab168603) -
Positive control
- This antibody gave a positive signal in Mouse Lung tissue lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab129820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB 1/250. Detects a band of approximately 115 kDa (predicted molecular weight: 103 kDa). Target
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Function
May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation. -
Tissue specificity
Highly expressed in heart. Detected at lower levels in placenta and pancreas. -
Sequence similarities
Contains 1 dilute domain.
Contains 1 Ras-associating domain. -
Cellular localization
Cytoplasm > perinuclear region. Golgi apparatus > Golgi stack. Associated with perinuclear vesicles. Is recruited to Golgi stacks by activated HRAS. - Information by UniProt
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Database links
- Entrez Gene: 54922 Human
- Entrez Gene: 69903 Mouse
- Omim: 609623 Human
- SwissProt: Q5U651 Human
- SwissProt: Q3U0S6 Mouse
- Unigene: 233955 Human
- Unigene: 181396 Mouse
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Alternative names
- FLJ20401 antibody
- Rain antibody
- RAIN protein fragment antibody
see all
Images
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Western blot - Anti-RAIN antibody (ab129820)Anti-RAIN antibody (ab129820) at 1/250 dilution +
Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 103 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Additional bands at: 23 kDa, 39 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The expression profile observed is consistent with what has been described in the literature PMID:15031288. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129820 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab129820 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-RAIN antibody (ab129820)Anti-RAIN antibody (ab129820) at 1/250 dilution +
Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 103 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Additional bands at: 23 kDa, 39 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The expression profile observed is consistent with what has been described in the literature PMID:15031288. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129820 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
