Anti-KLC4 antibody (ab101190)
Key features and details
- Rabbit polyclonal to KLC4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-KLC4 antibody -
Description
Rabbit polyclonal to KLC4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- This antibody gave a positive signal in Mouse and Rat brain tissue lysates as well as HeLa and Jurkat whole cell lysates.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatHumanAll applications CowDogMacaque monkeyApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 69 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 69 kDa).Target
-
Function
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity. -
Sequence similarities
Belongs to the kinesin light chain family.
Contains 7 TPR repeats. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
-
Database links
- Entrez Gene: 89953 Human
- Entrez Gene: 74764 Mouse
- Entrez Gene: 316226 Rat
- SwissProt: Q9NSK0 Human
- SwissProt: Q9DBS5 Mouse
- SwissProt: Q5PQM2 Rat
- Unigene: 655123 Human
- Unigene: 279599 Mouse
see all -
Alternative names
- Kinesin light chain 4 antibody
- kinesin like 8 antibody
- Kinesin like protein 8 antibody
see all
Images
-
Western blot - Anti-KLC4 antibody (ab101190)All lanes : Anti-KLC4 antibody (ab101190) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
The predicted molecular weight of KLC4 is 69 kDa (SwissProt), however we expect to observe a banding pattern at 75 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Datasheets and documents
References (0)
ab101190 has not yet been referenced specifically in any publications.
Images
-
Western blot - Anti-KLC4 antibody (ab101190)All lanes : Anti-KLC4 antibody (ab101190) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
The predicted molecular weight of KLC4 is 69 kDa (SwissProt), however we expect to observe a banding pattern at 75 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.