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Human Jagged1 ELISA Kit (ab267651)

Key features and details

  • Sensitivity: 1.6 ng/ml
  • Range: 1.638 ng/ml - 400 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Jagged1 ELISA Kit
    See all Jagged1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    overall
    Inter-assay
    Sample n Mean SD CV%
    overall

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.6 ng/ml

  • Range

    1.638 ng/ml - 400 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 77.24 69% - 90%
    Plasma 70.1 67% - 82%
    Cell culture media 99.66 75% - 127%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Jagged1 ELISA Kit is designed for the quantitative determination of Jagged1 in cell culture supernatants, plasma and serum samples.


    ?Note: Human Jagged1 concentration is low in normal serum/plasma, and may not be detectable in this assay.


    This assay employs an antibody specific for human Jagged1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Jagged1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Jagged1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Jagged1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    This ELISA kit detects human Jagged1. Other species not determined.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer 1 x 25ml
    250X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Anti-Human Jagged1 coated Microplate (12 x 8 wells) 1 unit
    Biotinylated Anti-Human Jagged1 Detection Antibody 2 vials
    Human Jagged1 Standard (Lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Solution 1 x 12ml

  • Research areas

  • Function

    Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).

  • Tissue specificity

    Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.

  • Involvement in disease

    Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
    Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.

  • Sequence similarities

    Contains 1 DSL domain.
    Contains 15 EGF-like domains.

  • Developmental stage

    Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • AGS
    • AHD
    • AWS
    • CD 339
    • CD339
    • CD339 antigen
    • Headturner
    • hJ1
    • Htu
    • Jag 1
    • Jag1
    • JAG1_HUMAN
    • Jagged 1
    • Jagged1
    • Jagged1 (Alagille syndrome)
    • JAGL1
    • MGC104644
    • OTTHUMP00000030278
    • Protein jagged-1
    • Ser 1
    • Ser1
    • Serrate 1
    • Slalom
    see all

  • Database links

    Images

    • Example data
      Example data

      This standard curve is for demonstration only. A standard curve must be run with each assay.

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