Human Jagged1 ELISA Kit (ab267651)
Key features and details
- Sensitivity: 1.6 ng/ml
- Range: 1.638 ng/ml - 400 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Jagged1 ELISA Kit
See all Jagged1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% overall Inter-assay Sample n Mean SD CV% overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.6 ng/ml -
Range
1.638 ng/ml - 400 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 77.24 69% - 90% Plasma 70.1 67% - 82% Cell culture media 99.66 75% - 127% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Jagged1 ELISA Kit is designed for the quantitative determination of Jagged1 in cell culture supernatants, plasma and serum samples.
?Note: Human Jagged1 concentration is low in normal serum/plasma, and may not be detectable in this assay.
This assay employs an antibody specific for human Jagged1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Jagged1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Jagged1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Jagged1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit detects human Jagged1. Other species not determined.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Human Jagged1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human Jagged1 Detection Antibody 2 vials Human Jagged1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro). -
Tissue specificity
Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells. -
Involvement in disease
Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent. -
Sequence similarities
Contains 1 DSL domain.
Contains 15 EGF-like domains. -
Developmental stage
Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- AGS
- AHD
- AWS
see all -
Database links
- Entrez Gene: 182 Human
- Omim: 601920 Human
- SwissProt: P78504 Human
- Unigene: 224012 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.
